Produced in E. coli. For production of poly-ubiquitin chains via Lys6 only. Lys6-only ubiquitin contains only a single lysine at position 6, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains only via Lys6. The ability to form an active thioester at the C-terminus is preserved, allowing Lys6-only ubiquitin to be transferred to the lysines of substrate proteins.
